Spotlight on USDA-ARS Scientists
For nearly ten years, scientists at Purdue University and the USDA-ARS Eastern Regional Research Center have established a strong partnership and have collaborated on a number of research and outreach projects. We are proud of the relationship that has continually grown since we joined forces in 1999. For this annual report, we highlight two USDA-ARS scientists that have been instrumental in fostering our overall CFSE effort.
Dr. Andrew Gehring
- Gehring, A.G., Albin, D.M., Bhunia, A., Reed, S.A., Tu, S., Uknalis, J. Antibody microarray detection of Escherichia coli O157:H7: Quantification, assay limitations, and capture efficiency. Analytical Chemistry. 2006. v. 78. p. 6601-6607.
- Gehring, A.G., Bailey, D.G., DiMaio, G.L., Crowther, J.C. Improved hide quality and rapid unhairing. Journal of the American Leather Chemists Association. 2002. v. 97. p. 339-348.
Dr. George Paoli
- Nanduria, V., Bhunia, A.K., Tu, S-I, Paoli, G.C., Brewster, J.D. SPR biosensor for the detection of L. monocytogenes using phage-displayed antibody. Biosensors and Bioelectronics. 2007. v. 23. p. 248-252.
- Paoli, G.C., Kleina, L.G., Brewster, J.D. Development of Listeria monocytogenes specific immunomagnetic beads using a single-chain antibody fragment. Foodborne Pathogens and Disease. 2007. v. 4. p. 74-83.
- Paoli, G.C., Bhunia, A.K., Bayles, D.O. Listeria monocytogenes. Fratamico, P.M., Bhunia, A.K., Smith J.L., editors. Caister Academic Press, Wymondham, Norfolk UK. Foodborne Pathogens: Microbiology and Molecular Biology. 2005. p. 295-325.
Dr. Gehring and Dr. Paoli
Since the inception of the Center for Food Safety Engineering, there has been a strong emphasis on interaction and collaboration with scientists from the UDSA-ARS Eastern Regional Research Center. This collaboration has been fostered through interaction between scientists during annual Purdue-CFSE/ARS-ERRC research planning workshops, annual participation by Purdue-CFSE and ARS-ERRC scientists in the Molecular Detection Symposium during the International Workshop on Rapid Methods and Automation in Microbiology at Kansas State University, and more recently, an invitation for Purdue-CFSE scientists to participate in an ARS-FSIS (Food Safety Inspection Service) research planning workshop, aimed at identifying specific FSIS needs.
In addition, ARS scientists have hosted Purdue students and post-doctoral fellows at ERRC who are conducting collaborative research. These collaborative research efforts have been presented at scientific meetings and have resulted in several publications in peer-reviewed journals.
Drs. Andrew Gehring and Arun Bhunia have interacted extensively on their mutual interest in the development of a mixed culture enrichment (Salmonella, E. coli O157:H7, Listeria monocytogenes, and Yersinia enterocolitica) in support of their work on multiplexed antibody-based microarray for detection of pathogenic bacteria from food. They have worked both independently and collaboratively on the development of the antibody-based microarray and have co-authored one publication on the subject. As part of her doctoral training, one of Dr. Bhunia's doctoral students, Kristen Nanchansky, spent a summer at ERRC working with Dr. Gehring developing a chemiluminescent immunoassay for the detection of L. monocytogenes. In addition, Drs. Bhunia and Gehring organized and co-chaired a symposium on Optical Technologies for Industrial, Environmental, and Biological Sensing-Food Safety and Agricultural Monitoring for the International Society for Optical Engineering Photonics East.
Drs. George Paoli and Arun Bhunia share an interest in studying the foodborne pathogen Listeria monocytogenes. Dr. Bhunia has developed and characterized a number of monoclonal antibodies against L. monocytogenes, and Dr. Paoli has applied antibody phage display to select a single-chain antibody specific to L. monocytogenes. In addition to co-authoring a chapter on the bacterium in a recently published book on the microbiology and molecular biology of foodborne pathogens, Drs. Paoli and Bhunia collaborated in supervising a post-doctoral study by Dr. Viswaprakash Nanduri on the development of a phage displayed antibody-based surface plasmon resonance optical biosensor for the detection of L. monocytogenes.
International Collaboration: CFSE goes to China
By invitation of Dr. Xianming Shi, the deputy director of the Bor-Luh Chinese Food Safety Center, Purdue scientists Arun Bhunia and Richard Linton joined USDA-ARS scientists (Shu-I Tu, Andrew Gehring, Yiping He, and George Paoli) and Dr. Jim Lindsay (ARS National Program Leader for Food Safety) in China in May 2008 to meet with representatives from Jiao-Tong University in Shanghai. At the meeting an agreement was signed forming a joint U.S.-Sino Food Safety Research Center between ARS and the Chinese Ministry of Science and Technology. We had a very productive meeting and already have plans to work together with USDA-ARS and Jiao-Tong University scientists in the near future. While in Shanghai, the group also attended and presented at the 10th World Congress on Biosensors.