Concentration of pathogenic bacteria from food samples

Investigator: Michael Ladisch (Department of Agricultural and Biological Engineering)

Project Report 2011 - 2012

Project Description:

Sample preparation methods that quickly concentrate the microorganisms present in a large food sample with minimal impact on their viability are an important first step in pathogen detection. The combined use of mechanical shearing and enzyme treatments enables rapid microfiltration of the bacteria through special membranes. The small volume of concentrated sample produced represents all of the bacteria present in the large food sample. This is important when the final detection method is only capable of analyzing small volumes, such as for PCR.

Project Highlight:

The concentration of pathogenic bacteria from food samples will allow for the rapid detection of the bacterial pathogens near the source so that interventions may be implemented sooner to reduce the distribution of unsafe food to consumers.

Annual Report